Recombinant Human Siglec-15 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9227-SL

R&D Systems, part of Bio-Techne
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9227-SL-050
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Key Product Details

Source

CHO

Accession #

Q6ZMC9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Siglec-15 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein
Human Siglec-15
(Phe20-Thr263)
Accession # Q6ZMC9		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20

Predicted Molecular Mass

53 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1-10 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9227-SL in the following applications:

Scientific Data Images for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Recombinant Human Siglec-15 Fc Chimera Protein Bioactivity

Recombinant Human Siglec-15 Fc Chimera Protein Bioactivity

Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.

Formulation, Preparation and Storage

9227-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

References

  1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
  2. Angata, T. et al. (2007) Glycobiology 17:838.
  3. Takamiya, R. et al. (2013) Glycobiology 23:178.
  4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Gene Symbol

SIGLEC15

UniProt

Q6ZMC9

Additional Siglec-15 Products

Product Documents for Recombinant Human Siglec-15 Fc Chimera Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Siglec-15 Fc Chimera Protein, CF

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