Recombinant Human Siglec-5 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1072-SL
Key Product Details
Source
Accession #
Structure / Form
Conjugate
Applications
Product Specifications
Source
Human Siglec-5 (Glu17-Thr434) Accession # O15389 |
IEGRID | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 1-4 µg/mL.
Formulation, Preparation and Storage
1072-SL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Siglec-5/CD170
Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11 (4, 5, 7). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.
Human Siglec-5 cDNA encodes a 551 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasma tail (6). Siglec-5 exists as a disulfide-linked homodimer on the cell surface and is expressed on monocytes, neutrophils and B cells (4, 5, 6). It binds equally well to both alpha2,3- and alpha2,6-linked sialic acid (6).
References
- Crocker, P.R. et al. (1998) Glycobiology 8:v.
- Powell, L.D. et al. (1995) J. Biol. Chem. 270:14243.
- May, A.R. et al. (1998) Mol. Cell 1998. 1:719.
- Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
- Crocker, P.R. et al. (2001) Immunology 103:137.
- Cornish, A.L. et al. (1998) Blood 92:2123.
- Angata, T. et al. (2002) J. Biol Chem. 277:24466.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Siglec-5/CD170 Products
Product Documents for Recombinant Human Siglec-5 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human Siglec-5 Fc Chimera Protein, CF
For research use only