Recombinant Human Siglec-8 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9045-SL

R&D Systems, part of Bio-Techne
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9045-SL-050
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Key Product Details

Source

NS0

Accession #

AAH53319

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Siglec-8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Siglec-8 protein
Human Siglec-8
(Met17-Ala363)
Accession # AAH53319		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met17

Predicted Molecular Mass

64 kDa

SDS-PAGE

68-88 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Sialyl Lewis X was immobilized at 0.05 μg/mL (100 μL/well), it binds Recombinant Human Siglec-8 Fc Chimera. The concentration of Human Siglec-8 Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.

Formulation, Preparation and Storage

9045-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-8

Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs (2, 3). Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs (4, 5). Siglec-8 is expressed on eosinophils, basophils, and mast cells (2, 3), and it shows a binding preference for the carbohydrate 6-O sulfated sLex (3, 6, 7). In mouse, Siglec-F is a functional paralog of human Siglec-8, as shown by its similar expression profile and ligand specificity (8). Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF (10-12).

References

  1. Kiwamoto, T. et al. (2013) Curr. Opin. Allergy Clin. Immunol. 13:106.
  2. Kikly, K.K. et al. (2000) J. Allergy Clin. Immunol. 105:1093.
  3. Floyd, H. et al. (2000) J. Biol. Chem. 275:861.
  4. Aizawa, H. et al. (2002) J. Allergy Clin. Immunol. 109:176.
  5. Foussias, G. et al. (2000) Biochem. Biophys. Res. Commun. 278:775.
  6. Bochner, B.S. et al. (2005) J. Biol. Chem. 280:4307.
  7. Hudson, S.A. et al. (2011) J. Clin. Immunol. 31:1045.
  8. Tateno, H. et al. (2005) Glycobiology 15:1125.
  9. Yokoi, H. et al. (2008) J. Allergy Clin. Immunol. 121:499.
  10. Kano, G. et al. (2013) J. Allergy Clin. Immunol. 132:437.
  11. Nutku, E. et al. (2003) Blood 101:5014.
  12. Na, H.J. et al. (2012) Cytokine 57:169.

Long Name

Sialic Acid Binding Ig-like Lectin 8

Alternate Names

SAF2, Siglec8, SIGLEC8L

Entrez Gene IDs

27181 (Human)

Gene Symbol

SIGLEC8

UniProt

AAH53319

Additional Siglec-8 Products

Product Documents for Recombinant Human Siglec-8 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Siglec-8 Fc Chimera Protein, CF

For research use only

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