Recombinant Human SIRP alpha/CD172a His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9378

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9378-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P78324.2

Structure / Form

Monomer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all SIRP alpha/CD172a Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein
Human SIRP alpha/CD172a
(Glu31-Asn371)
Accession # P78324.2		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31

Predicted Molecular Mass

40 kDa

SDS-PAGE

49-62 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRP alpha/CD172a His-tag Avi-tag (Catalog # AVI9378) binds with an ED50 of 0.2-1.4 μg/mL.

Scientific Data Images for Recombinant Human SIRP alpha/CD172a His Avi-tag Protein, CF

Recombinant Human SIRP alpha/CD172a His Avi-tag Protein Binding Activity

Recombinant Human SIRP alpha/CD172a His Avi-tag Protein Binding Activity

When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRPa/CD172a His-tag Avi-tag (Catalog # AVI9378) binds with an ED50 of 0.2-1.4 µg/mL.
Recombinant Human SIRP alpha/CD172a His Avi-tag Protein SDS-PAGE

Recombinant Human SIRP alpha/CD172a His Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human SIRP alpha/CD172a His-tag Avi-tag (Catalog # AVI9378) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-62 kDa.

Formulation, Preparation and Storage

AVI9378
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP alpha/CD172a

Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1-4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha2 or PTPNS) (5). One reported isoform lacks aa 1-101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta1 and SIRP gamma, respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3-6). It is also found on neurons, smooth muscle and endothelial cells (7-9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).

References

  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. Liu, Y. et al. (2005) J. Biol. Chem. 280:36132.
  4. Kharitonenkov, A. et al. (1997) Nature 386:181.
  5. Swissprot Accession # P7832.
  6. Miyashita, M. et al. (2004) Mol. Biol. Cell 15:3950.
  7. Wang, X.X. & K.H. Pfenninger (2005) J. Cell Sci. 119:172.
  8. Maile, L.A. et al. (2003) Mol. Biol. Cell 14:3519.
  9. Johansen, M.L. & E.J. Brown (2007) J. Biol. Chem. 282:24219.
  10. Takenaka, K. et al. (2007) Nat. Immunol. 8:1313.
  11. Ishikawa-Sekigami, T. et al. (2006) Biochem. Biophys. Res. Commun. 343:1197.
  12. Olsson, M. et al. (2005) Blood 105:3577.
  13. Ide, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5062.
  14. Gardai, S.J. et al. (2003) Cell 115:13.
  15. Lundberg, P. et al. (2007) Biochem. Biophys. Res. Commun. 352:444.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Gene Symbol

SIRPA

UniProt

P78324.2

Additional SIRP alpha/CD172a Products

Product Documents for Recombinant Human SIRP alpha/CD172a His Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human SIRP alpha/CD172a His Avi-tag Protein, CF

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