Recombinant Human SIRP beta 1/CD172b Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9978-SB

R&D Systems, part of Bio-Techne
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9978-SB-050
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Key Product Details

Source

HEK293

Structure / Form

Dimer, non covalent

Conjugate

Unconjugated

Applications

Bioactivity

View all SIRP beta 1/CD172b Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SIRP beta 1/CD172b protein
Human SIRP beta 1/CD172b
(Glu30-Ala369)
Accession # O00241
 HH
 Hemagglutinin Tag
(YPYDVPDYA)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu30

Predicted Molecular Mass

38 kDa

SDS-PAGE

45-61 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SIRP beta1/CD172b (Catalog # 9978-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 0.1-0.8 μg/mL

Scientific Data Images for Recombinant Human SIRP beta 1/CD172b Protein, CF

Recombinant Human SIRP beta 1/CD172b Protein Binding Activity

Recombinant Human SIRP beta 1/CD172b Protein Binding Activity

When Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) is immobilized at 1 µg/mL, Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 0.1-0.8 µg/mL.
Recombinant Human SIRP beta 1/CD172b Protein SDS-PAGE

Recombinant Human SIRP beta 1/CD172b Protein SDS-PAGE

2 μg/lane of Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-61 kDa, respectively.

Formulation, Preparation and Storage

9978-SB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1/CD172b

Signal-regulatory protein beta 1 (SIRP beta 1) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. The extracellular domain contains 3 intramolecular disulfide bonds and one interchain disulfide at Cys320. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Human SIRP beta 1 shares a 57% sequence identity with mouse and rat SIRP beta 1. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5,6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

References

  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich J. et al. (2000) J Immunol. 164:9.
  6. Brook G. et al. (2004) J Immunol. 173:2562.
  7. Hayashi A. et al. (2004) J Biol Chem. 279:29450.
  8. Fournier B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert M. et al. (2001) Blood 97:2741.

Long Name

Signal-regulatory Protein beta 1

Alternate Names

CD172b, SIRPB1

Entrez Gene IDs

10326 (Human); 102139733 (Cynomolgus Monkey)

Gene Symbol

SIRPB1

Additional SIRP beta 1/CD172b Products

Product Documents for Recombinant Human SIRP beta 1/CD172b Protein, CF

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Product Specific Notices for Recombinant Human SIRP beta 1/CD172b Protein, CF

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