Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9998-SB

R&D Systems, part of Bio-Techne
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9998-SB-050
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Key Product Details

Source

HEK293

Accession #

Q5JXA9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all SIRP beta 2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SIRP beta 2 protein
          
Human SIRP beta 2
(Gln33-Gly287)
Accession # Q5JXA9		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Ser34.

Predicted Molecular Mass

55 kDa

SDS-PAGE

77-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SIRP beta 2 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD300B/LIMR5 Fc Chimera binds with an ED50 of 2-10 μg/mL.

Scientific Data Images for Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

Recombinant Human SIRP beta 2 Fc Chimera Protein Binding Activity

Recombinant Human SIRP beta 2 Fc Chimera Protein Binding Activity

When Recombinant Human SIRP beta 2 Fc Chimera (Catalog # 9998-SB) is immobilized at 1 µg/mL, Biotinylated Recombinant CD300B/LMIR-5 Fc Chimera binds with an ED50 of 2-10 μg/mL.
Recombinant Human SIRP beta 2 Fc Chimera Protein SDS-PAGE

Recombinant Human SIRP beta 2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human SIRP beta 2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-85 kDa and 150-170 kDa, respectively.

Formulation, Preparation and Storage

9998-SB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References

  1. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
  3. Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
  4. Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
  5. Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
  6. Veillette A. (2018) Trends Immunol. 39:173.

Long Name

Signal-regulatory Protein beta 2

Alternate Names

PTPN1L, PTPNS1L3

Entrez Gene IDs

284759 (Human)

Gene Symbol

SIRPB2

UniProt

Q5JXA9

Additional SIRP beta 2 Products

Product Documents for Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

For research use only

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