Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9999

Biotinylated His-tag
R&D Systems, part of Bio-Techne
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AVI9999-050
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Key Product Details

Source

CHO

Accession #

Q9P1W8.3

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all SIRP gamma/CD172g Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SIRP gamma/CD172g protein
Human SIRPG
(Glu29-Pro360)
Accession # Q9P1W8.3		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu29

Predicted Molecular Mass

39 kDa

SDS-PAGE

42-49 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human SIRP gamma/CD172g His-tag Avi-tag (Catalog # AVI9999) binds Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) with an ED50 of 0.150-3.00 μg/mL.

Scientific Data Images for Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

Biotinylated Recombinant Human SIRP gamma/CD172g His-tag Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human SIRP gamma/CD172g His-tag Avi-tag Protein (Catalog # AVI9999) binds Recombinant Human CD47 Fc Chimera (4670-CD) with an ED50 of 0.150-3.00 μg/mL.

Biotinylated Recombinant Human SIRP gamma/CD172g His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human SIRP gamma/CD172g His-tag Avi-tag Protein (Catalog # AVI9999) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-49 kDa, under reducing conditions.

Formulation, Preparation and Storage

AVI9999
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP gamma/CD172g

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C-terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5-7). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling motif (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T-cell and monocyte cell lines (6). Our Avi-tag Biotinylated Recombinant Human SIRP gamma/CD172g His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
  5. Piccio, L. et al. (2005) Blood 105:2421.
  6. Brooke, G. et al. (2004) J. Immunol. 173:2562.
  7. Smith, M.J. et al. (2022) Diabetes. 71:350.

Long Name

Signal-regulatory Protein gamma

Alternate Names

CD172g, SIRP beta 2, SIRPG

Entrez Gene IDs

55423 (Human); 102138723 (Cynomolgus Monkey)

Gene Symbol

SIRPG

UniProt

Q9P1W8.3

Additional SIRP gamma/CD172g Products

Product Documents for Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

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