Recombinant Human SLITRK2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8947-SK

R&D Systems, part of Bio-Techne
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8947-SK-050
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Key Product Details

Source

NS0

Accession #

Q9H156

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLITRK2 protein
Thr19-Pro617, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr19

Predicted Molecular Mass

68 kDa

SDS-PAGE

80-100 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

8947-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK2

SLITRK2 (Slit and Trk-like family member 2) is an approximately 110 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK2 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK2 shares 98% amino acid sequence identity with mouse and rat SLITRK2. SLITRK2 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (3-5). The synaptogenic function is dependent on the interaction of SLITRK2 with select isoforms of PTP-sigma (5, 6). In humans, mutations of SLITRK6 are associated with bipolar disorder and schizophrenia (7, 8).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Yamagata, A. et al. (2015) Sci. Rep. 5:9686.
  7. Smith, E.N. et al. (2009) Mol. Pschiatry 14:755.
  8. Piton, A. et al. (2011) Mol. Pschiatry 16:867.

Long Name

SLIT and NTRK-like Family, Member 2

Alternate Names

CXorf2, SLITL1

Entrez Gene IDs

84631 (Human); 245450 (Mouse)

Gene Symbol

SLITRK2

UniProt

Q9H156

Additional SLITRK2 Products

Product Documents for Recombinant Human SLITRK2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SLITRK2 Protein, CF

For research use only

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