Recombinant Human SLITRK3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8957-SK

R&D Systems, part of Bio-Techne
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8957-SK-050
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Key Product Details

Source

NS0

Accession #

O94933

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLITRK3 protein
Thr29-Val651, with substitutions of Arg425Ser, Asn490Tyr, Phe510Leu and a C-terminal 6-His tag

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr29

Predicted Molecular Mass

71 kDa

SDS-PAGE

80-108 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

8957-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK3

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK3 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK3 shares 98% amino acid sequence identity with mouse and rat SLITRK3. It is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-5). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is upregulated during the development of gastrointestinal stromal tumors (6).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Wang, C.-J. et al. (2015) World J. Gastroenterol. 21:8398.

Long Name

SLIT and NTRK-like Family, Member 3

Alternate Names

MGC138681, SLIT and NTRK-like family, member 3

Entrez Gene IDs

22865 (Human); 386750 (Mouse); 310519 (Rat)

Gene Symbol

SLITRK3

UniProt

O94933

Additional SLITRK3 Products

Product Documents for Recombinant Human SLITRK3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human SLITRK3 Protein, CF

For research use only

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