Recombinant Human SLITRK4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8945-SK

R&D Systems, part of Bio-Techne
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8945-SK-050
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Key Product Details

Source

NS0

Accession #

Q8IW52

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SLITRK4 protein
Asp19-Pro616, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp19

Predicted Molecular Mass

68 kDa

SDS-PAGE

87-105 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 90 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

8945-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK4

SLITRK4 (Slit and Trk-like family member 4) is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK4 shares 98% amino acid sequence identity with mouse and rat SLITRK4. It is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3-5).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.

Long Name

SLIT and NTRK-like Family, Member 4

Alternate Names

DKFZp547M2010, SLIT and NTRK-like family, member 4, SLIT and NTRK-like protein 4, slit and trk like gene 4

Entrez Gene IDs

139065 (Human); 245446 (Mouse)

Gene Symbol

SLITRK4

UniProt

Q8IW52

Additional SLITRK4 Products

Product Documents for Recombinant Human SLITRK4 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human SLITRK4 Protein, CF

For research use only

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