Recombinant Human SorLA His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11083-LA

R&D Systems, part of Bio-Techne
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11083-LA-050
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Key Product Details

Source

CHO

Accession #

NP_003096.1

Conjugate

Unconjugated

Applications

Bioactivity

View all SorLA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SorLA protein
Ser82-Asp2135, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser82

Predicted Molecular Mass

232 kDa

SDS-PAGE

270-345 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SorLA His-tag Protein is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (Catalog # 220-BB). The ED50 for this binding is 2.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human SorLA His-tag Protein, CF

Recombinant Human SorLA His-tag Protein Binding Activity.

When Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (220-BB). The ED50 for this binding is 2.00-30.0 ng/mL.

Recombinant Human SorLA His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 270-345 kDa.

Formulation, Preparation and Storage

11083-LA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorLA

SorLA (sortilin-related receptor, LDLR class A repeats-containing), also called LR11 or SORL1, is a 250 kDa type I transmembrane glycoprotein of the Sortilin family of Vps10p-domain receptors (1, 2). SorLA is found in both intracellular and surface membranes in the central nervous system, and mediates trafficking of proteins such as the amyloid precursor protein (APP) (3-5). The 2214 amino acid (aa) human SorLA precursor includes a 28 aa signal sequence and a 53 aa furin-cleaved propeptide. The mature SorLA contains a vacuolar protein sorting 10 protein (Vps10p) homology domain, beta-propeller and epidermal growth factor (EGF) domains, a cluster of 11 complement-type repeat domains, six fibronectin type III repeats, a single transmembrane domain (TM), and a cytoplasmic domain (CT) (1, 6-7). Human SorLA shares 93% and 92% aa sequence identity with mouse and rat SorLA, respectively. SorLA is mainly expressed in nervous system, but is also found in non-neuronal tissues (8). Upon truncation, SorLA is able to bind multiple ligands among which are RAP, apolipoprotein E, and lipoprotein lipase, and facilitate both their endocytosis and sorting (1). The expression, translocation, and ectodomain shedding were shown to be induced by its ligand head activator peptide (HA) (9). SorLA has also been shown to be important in Alzheimer's disease through multiple functions, but especially through its ability to bind Amyloid Precursor Protein (4). It is involved in TrkB receptor and GFRa1 trafficking and further enhancement of BDNF and GDNF functions respectively (10, 11).

References

  1. Jacobsen, L. et al. (2001) J. Biol. Chem. 276:22788.
  2. Wang, X. et al. (2011) Protein Expr. Purif. 77:224.
  3. Small, S.A. and S. Gandy (2006) Neuron. 52:15.
  4. Andersen, O.M. et al. (2005) Proc. Natl. Acad. Sci. U. S. A. 102:13461.
  5. Willnow, T.E. et al. (2008) Nat. Rev. Neurosci. 9:899.
  6. Herz J. (2001) Neuron 29:571.
  7. Yamazaki H. et al. (1996) J. Biol. Chem. 271:24761.
  8. Jacobsen L. et al. (1996) J. Biol. Chem. 271:31379.
  9. Hampe, W. et al. (2000) J. Cell Sci. 113:4475.
  10. Rohe, M. et al. (2013) PLoS One. 8:72164.
  11. Glerup, S. et al. (2013) Cell Rep. 3:186.

Long Name

Sortilin-related Receptor Containing LDLR Class A Repeats

Alternate Names

gp250, LR11, LRP9, SORL1

Entrez Gene IDs

6653 (Human); 20660 (Mouse); 300652 (Rat)

Gene Symbol

SORL1

UniProt

NP_003096.1 (Human)

Additional SorLA Products

Product Documents for Recombinant Human SorLA His-tag Protein, CF

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Product Specific Notices for Recombinant Human SorLA His-tag Protein, CF

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