Recombinant Human ST3GAL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6905-GT

R&D Systems, part of Bio-Techne
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6905-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

Q11201

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 protein
Arg57-Arg340, with an N-terminal HA (YPYDVPDYA) tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (of HA tag)

Predicted Molecular Mass

33.4 kDa

SDS-PAGE

35-45 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine B1-3 galactosyl-N-acetyl galactosamine.
The specific activity is >1000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6905-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL1 is a type II membrane protein localized in the trans‑Golgi network that transfers sialic acid to galactose residues of core 1 O‑glycans (3Gal beta1‑3GalNAc) to produce sialyl‑T antigen (NeuAc alpha2‑3Gal beta1‑3GalNAc) (2, 3). ST3GAL1 is ubiquitously expressed in most tissues, with highest expression in the placenta, liver and skeletal muscle (4). Over‑expression of ST3GAL1 may be involved in the tumorigenesis of both breast cancer (5, 6) and bladder cancer (7). The enzyme activity of the recombinant human ST3GAL1 was determined using a phosphatase‑coupled glycosyltransferase assay (8).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
  3. Dalziel, M. et al. (2001) J. Biol. Chem. 276:11007.
  4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
  5. Picco, G. et al. (2010) Glycobiology 20:1241.
  6. Burchell, J. et al. (1999) Glycobiology 9:1307.
  7. Videira, P.A. et al. (2009) BMC Cancer 9:357.
  8. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1

Alternate Names

Gal-NAc6S, SIAT4A, SIATFL, ST3GalA, ST3O

Entrez Gene IDs

6482 (Human); 20442 (Mouse); 362924 (Rat)

Gene Symbol

ST3GAL1

UniProt

Q11201

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Products

Product Documents for Recombinant Human ST3GAL1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST3GAL1 Protein, CF

For research use only

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