Recombinant Human ST6GAL1 (aa 44-406) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7620-GT

R&D Systems, part of Bio-Techne
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7620-GT-010
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

P15907

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST6 Gal Sialyltransferase 1/ST6GAL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST6 Gal Sialyltransferase 1/ST6GAL1 protein
Glu44-Cys406 with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

42 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 7620-GT in the following applications:

Formulation, Preparation and Storage

7620-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

Sialic acid molecules attached to glycoproteins or glycosphingo lipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1).Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B‑cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9). Using a phosphatase-coupled sialyltransferase assay, the Km for the donor substrate CMP-NeuAc was determined to be around 0.5 mM (10).

References

  1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
  2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
  4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
  5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
  6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
  7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
  8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  9. Kitazume, S. et al. (2009) Glycobiology 19:479.
  10.  Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Entrez Gene IDs

6480 (Human); 20440 (Mouse); 25197 (Rat)

Gene Symbol

ST6GAL1

UniProt

P15907

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

Product Documents for Recombinant Human ST6GAL1 (aa 44-406) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ST6GAL1 (aa 44-406) Protein, CF

For research use only

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