Recombinant Human ST6GALNAC4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6876-GT

R&D Systems, part of Bio-Techne
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6876-GT-010
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

Q9H4F1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST6GALNAC4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST6GALNAC4 protein
Thr36-Arg302, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr36 and Leu38

Predicted Molecular Mass

31 kDa

SDS-PAGE

36-38 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >1000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6876-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST6GALNAC4

ST6GALNAC4 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates (1). This enzyme works on both glycoproteins and glycolipids and has strict substrate specificity, utilizing only the trisaccharide sequence Neu5Ac alpha2-3Gal beta1-3GalNAc of glycoproteins and glycolipids (2). In particular, this enzyme is involved in the synthesis of ganglioside GD1A from GM1B (3). GD1A is the target pathogenic antigen in the autoimmune disease, Guillain-Barre Syndrome (4, 5). The activity of this enzyme has been measured using a phosphatase-coupled method (6).

References

  1. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  2. Harduin-Lepers, A. et al. (2000) Biochem. J. 352:37.
  3. Lee, Y. C. et al. (1999) J. Biol. Chem. 274:11958.
  4. Kusunoki, S. et al. (1994) Ann. Neurol. 35:570.
  5. Goodfellow, J.A. et al. (2005) J. Neurosci. 25:1620.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

SIAT3C, SIAT7D

Entrez Gene IDs

27090 (Human); 20448 (Mouse)

Gene Symbol

ST6GALNAC4

UniProt

Q9H4F1

Additional ST6GALNAC4 Products

Product Documents for Recombinant Human ST6GALNAC4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST6GALNAC4 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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