Recombinant Human ST6GALNAC5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6715-GT

R&D Systems, part of Bio-Techne
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6715-GT-020
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Key Product Details

Source

CHO

Accession #

Q9BVH7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 protein
Gly30-Phe336, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

36 kDa

SDS-PAGE

40-55 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >40 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6715-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha2,3Gal beta1,3GalNAc beta1,4(NeuAc alpha2,3)Gal beta1,4Glc beta1-Cer) from GM1b (NeuAc alpha2,3Gal beta1,3GalNAc beta1,4Gal beta1,4Glc beta1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.

References

  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
  3. Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
  4. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  5. Bos, P.D. et al. (2009) Nature 459:1005.
  6. Arshad, F. et al. (2011). Patholog. Res. Int. Doi: 10.4061/2011/920509.
  7. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Long Name

ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5

Alternate Names

GD1 alpha synthase, SIAT7E, ST6GalNAcV

Entrez Gene IDs

81849 (Human); 26938 (Mouse)

Gene Symbol

ST6GALNAC5

UniProt

Q9BVH7

Additional ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Products

Product Documents for Recombinant Human ST6GALNAC5 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ST6GALNAC5 Protein, CF

For research use only

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