Recombinant Human ST8SIA4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7027-GT

R&D Systems, part of Bio-Techne
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7027-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

Q92187

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 protein
Gly40-Gln359, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

37 kDa

SDS-PAGE

55-66 kDa, reducing conditions

Activity

Measured by its ability to transfer sialic acid from CMP-NeuAc to Recombinant Human NCAM-1/CD56 120 isoform (Catalog # 2408-NC).
The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7027-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4

Polysialic acid (PSA), a glycan abundant on the neural cell adhesion molecule (NCAM) during embryonic development, negatively modulates the adhesive properties of NCAM (1). Following birth, PSA expression decreases promptly and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb-areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). While ST8SIA2 shows strict preference for NCAM (6), ST8SIA4 is also active on other glycoproteins, such as fetuin, in vitro (7). Unlike ST8SIA2, ST8SIA4 can catalyze both the initiation and polymerization of PSA. A combination of ST8SIA2 and ST8SIA4 cooperatively polysialylate NCAM, resulting in PSA with longer chains than when the enzymes are used individually (8). Disruption of ST8SIA4 in mice leads to impaired long-term potentiation and long-term depression of synapses of the adult hippocampus, similar to the phenotypes observed in NCAM‑deficient mice (9). Like most glycosyltransferases, ST8SIA4 may be a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (10).

References

  1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
  2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
  3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23:5908.
  4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
  5. Ong, E. et al. (1998). Glycobiology 8:415.
  6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
  7. Nakayama, J. and Fukuda, M. (1996) J. Biol. Chem. 271:1829.
  8. Angata, K. and Fukuda, M. (2002) J. Biol. Chem. 277:36808.
  9. Eckhardt, M. et al. (2000) J. Neurosci. 20:5234.
  10. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4

Alternate Names

PST1, SIAT8-D, ST8SIAIV

Entrez Gene IDs

7903 (Human); 20452 (Mouse)

Gene Symbol

ST8SIA4

UniProt

Q92187

Additional ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Products

Product Documents for Recombinant Human ST8SIA4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST8SIA4 Protein, CF

For research use only

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