Recombinant Human SULT1A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5546-ST

R&D Systems, part of Bio-Techne
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5546-ST-020
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Key Product Details

Source

E. coli

Accession #

P50225

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 1A1/SULT1A1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 1A1/SULT1A1 protein
Glu2-Leu295, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

35 kDa

SDS-PAGE

34 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to 1-Napthol.
The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5546-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 1A1/SULT1A1

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2).  SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity (2). The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters (3). Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers (2). The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility (4). The enzymatic activity of the recombinant human SULT1A1 is measured using a phosphatase-coupled assay (5).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Wilborn, T.W. et al. (1993) Mol. Pharm. 43:70.
  4. Gamage, N. U. et al. (2003) J. Biol. Chem. 278:7655.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

P-PST, PST, ST1A3, STP1, SULT1A1, TSPST1

Entrez Gene IDs

6817 (Human); 20887 (Mouse); 83783 (Rat)

Gene Symbol

SULT1A1

UniProt

P50225

Additional Cytosolic Sulfotransferase 1A1/SULT1A1 Products

Product Documents for Recombinant Human SULT1A1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SULT1A1 Protein, CF

For research use only

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