Recombinant Human SULT2A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5828-ST

R&D Systems, part of Bio-Techne
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5828-ST-020
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Key Product Details

Source

E. coli

Accession #

Q06520

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 2A1/SULT2A1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 2A1/SULT2A1 protein
Ser2-Glu285, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

35 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to t-Dehydroandrosterone (DHEA).
The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5828-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Cytosolic sulfotransferases are a family of Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important roles in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. They are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchor domains and residing only in the cytoplasm. SULT2A1 is primarily expressed in liver and adrenal tissues where it sulfates steroids and bile acids (4‑6). It is also called DHEA/bile acid sulfotransferase and plays roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and, at the same time, preventing toxicity from bile acid accumulation. The enzymatic activity of the recombinant human SULT2A1 is measured using a phosphatase-coupled assay (7).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
  4. Comer, K. A. et al. (1993) Biochem. J. 289:233.
  5. Otterness, D.M. et al. (1992) Mol. Pharmacol. 41:865.
  6. Luu-The, V. et al. (1995) DNA Cell Biol. 14: 511.
  7. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1

Entrez Gene IDs

6822 (Human); 20859 (Mouse); 24912 (Rat)

Gene Symbol

SULT2A1

UniProt

Q06520

Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products

Product Documents for Recombinant Human SULT2A1 Protein, CF

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Product Specific Notices for Recombinant Human SULT2A1 Protein, CF

For research use only

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