Recombinant Human SULT2B1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6174-ST

R&D Systems, part of Bio-Techne
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6174-ST-020
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Key Product Details

Source

E. coli

Accession #

O00204

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 2B1/SULT2B1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 2B1/SULT2B1 protein
Met1-Glu311, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

36 kDa

SDS-PAGE

34-37 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to pregnenolone.
The specific activity is >10 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6174-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol, Brij and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 2B1/SULT2B1

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). Compared to other cytosolic sulfotransferase, SULT2B1 is unique in that it contains a Pro‑rich region between amino acid 305 to 364 that may be related to the thermostability and nuclear translocation of the enzyme (3). The gene for SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini (4). Our recombinant enzyme corresponds to SULT2B1b. SULT2B1b preferentially sulfonates cholesterol and is less active on pregnenolone, DHEA and androstenediol; whereas SULT2B1a preferentially sulfonates pregnenolone and has minimal activity on cholesterol (5). The enzyme activity was determined using a phosphatase-coupled method (6).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. He, D. and Falany, C. N. (2006) Drug Metab. Dispos. 34:1749.
  4. Her, C. et al. (1998) Genomics. 53:284.
  5. Fuda, H. et al. (2002) J. Biol. Chem. 277:36361.
  6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

HSST2, SULT2B1

Entrez Gene IDs

6820 (Human); 54200 (Mouse); 292915 (Rat)

Gene Symbol

SULT2B1

UniProt

O00204 (Human)

Additional Cytosolic Sulfotransferase 2B1/SULT2B1 Products

Product Documents for Recombinant Human SULT2B1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human SULT2B1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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