Recombinant Human TFF1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8289-TF

R&D Systems, part of Bio-Techne
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8289-TF-050
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Key Product Details

Source

CHO

Accession #

P04155

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TFF1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TFF1 protein
Glu25-Phe84 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

7.5 kDa

SDS-PAGE

12-15 kDa, reducing conditions

Activity

Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells.
5-15 μg/mL of Recombinant Human TFF1 can effectively induce ERK1/2 phosphorylation.

Formulation, Preparation and Storage

8289-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF1

Trefoil Factor 1 (TFF1), also known as pS2, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF1 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). Mature human TFF1 shares 67% amino acid sequence identity with mouse and rat TFF1. It is expressed by goblet cells of the gastric and intestinal mucosa and by conjunctival goblet cells (2-5). TFF1 is a copper-binding protein that can form disulfide-linked homodimers, associate into disulfide-linked complexes with Gastrokine 2, and form non-covalent complexes with the mucin MUC5AC (4, 6-8). Copper enhances TFF1 homodimerization as well as its ability to promote epithelial cell motility, wound healing, and the colonization of H. pylori in stomach and colon epithelia (9, 10). TFF1 is down-regulated during the progression from gastritis to gastric dysplasia to gastric cancer, although it is up-regulated in breast and prostate cancers (11-13). TFF1 inhibits the formation of calcium oxalate crystals, and its excretion in the urine is reduced in patients with kidney stones (14).

References

  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505.
  3. Rio, M.C. et al. (1988) Science 241:705.
  4. Newton, J.L. et al. (2000) Gut 46:312.
  5. Langer, G. et al. (1999) Invest. Opthalmol. Visual Sci. 40:2220.
  6. Chadwick, M.P. et al. (1997) Biochem. Biophys. Res. Commun. 327:117.
  7. Westley, B.R. et al. (2005) Biochemistry 44:7967.
  8. Ruchaud-Sparagano, M.H. et al. (2004) Cell. Mol. Life Sci. 61:1946.
  9. Tosco, A. et al. (2010) Cell. Mol. Life Sci. 67:1943.
  10. Montefusco, S. et al. (2013) PLoS ONE 8:e79455.
  11. Soutto, M. et al. (2011) J. Clin. Invest. 121:1753.
  12. Kida, N. et al. (1989) Cancer Res. 49:3494.
  13. Vestergaard, E.M. et al. (2006) Clin. Cancer Res. 12:807.
  14. Chutipongtanate, S. et al. (2005) J. Clin. Invest. 115:3613.

Long Name

Trefoil Factor 1

Alternate Names

BCEI, D21S21, HPS2, PNR-2, PS2

Entrez Gene IDs

7031 (Human); 100041004 (Mouse); 117270 (Rat)

Gene Symbol

TFF1

UniProt

P04155

Additional TFF1 Products

Product Documents for Recombinant Human TFF1 Protein, CF

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Product Specific Notices for Recombinant Human TFF1 Protein, CF

For research use only

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