Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein

R&D Systems, part of Bio-Techne | Catalog # Qk010

Human/Bovine/Porcine
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Qk010-0050
Qk010-0500
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P01137.2

Conjugate

Unconjugated

Applications

Bioactivity

View all TGF-beta 1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived TGF-beta 1 protein

Purity

Single species with expected mass

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

24 kDa (dimer)

SDS-PAGE

Dimeric TGF- beta1 PLUS protein only

Activity

No significant difference between EC50 of reference and test lots

Mycoplasma

Negative when tested in both ribosomal RNA hybridization and luminescence assays

Scientific Data Images for Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein

Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein Bioactivity

TGF-beta 1 PLUS activity is determined using the firefly luciferase reporter assay (*) in transiently transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of TGF-beta 1. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity. EC50 = 34.8 pg/ml (1.5 pM). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein SDS-PAGE

TGF-beta 1 PLUS (Qk010) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form).Purified recombinant protein (7 μg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Formulation, Preparation and Storage

Qk010
Formulation Lyophilized from acetonitrile/TFA
Reconstitution Resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired.
Shipping The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below.
Stability & Storage Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Background: TGF-beta 1

TGF‑ beta1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta1 shares 100% aa identity with pig, dog and cow TGF‑ beta1, and 99% aa identity with mouse, rat and horse TGF‑ beta1. It demonstrates cross‑species activity (1). TGF‑ beta1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

UniProt

P01137.2

Additional TGF-beta 1 Products

Product Documents for Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TGF-beta 1 PLUS, Animal-Free Protein

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.