Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI3025

Biotinylated
R&D Systems, part of Bio-Techne
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AVI3025-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P36897.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity

View all TGF-beta RI/ALK-5 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta RI/ALK-5 protein
Human TGF-beta R1/ALK-5
(Ala25 or Leu34-Glu125)
Accession # P36897.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25, Leu34

Predicted Molecular Mass

39 kDa

SDS-PAGE

43-60 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Recombinant Human (rh) TGF-beta RII Fc Chimera  (Catalog # 341-BR) is immobilized at 1 μg/mL (100 μL/well), in the presence of rhTGF‑ beta1 (Catalog # 240-B), Biotinylated Recombinant Human TGF-beta  RI/ALK-5 Fc Chimera Avi-tag protein binds with an ED50 of 0.15-0.75 μg/mL.

Scientific Data Images for Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag Binding Activity.

When Recombinant Human TGF-beta RII Fc Chimera (341-BR) is immobilized at 1 μg/mL (100 μL/well), in the presence of Recombinant Human TGF-beta 1 (240-B), it binds Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag (Catalog # AVI3025) with an ED50 of 0.15-0.75 μg/mL.

Biotinylated Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag (Catalog # AVI3025) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-60 kDa and 86-120 kDa, respectively

Formulation, Preparation and Storage

AVI3025
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, human TGF-beta RI shares 90% and 88% aa sequence identity with mouse and rat TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9). Our Avi-tg Biotinylated human TGF-beta RI features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.



References

  1. Wrana, J.L. et al. (1994) Nature 370:341.
  2. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
  3. Huse, M. et al. (1999) Cell 96:425.
  4. Marcías-Silva, M. et al. (1996) Cell 87:1215.
  5. Zhang, Y. et al. (1996) Nature 383:168.
  6. Huse, M. et al. (2001) Mol. Cell 8:671.
  7. Larsson, J. et al. (2001) EMBO J. 20:1663.
  8. James, J.M. et al. (2013) Development 140:3903.
  9. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

P36897.1

Additional TGF-beta RI/ALK-5 Products

Product Documents for Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF

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