Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11124

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11124-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q495A1.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all TIGIT Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TIGIT protein
Human TIGIT
(Met22-Gln139, T103)
Accession # Q495A1.1		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22

Predicted Molecular Mass

21 kDa

SDS-PAGE

18-29 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD155/PVR His Protein (Catalog # 2530-CD) is immobilized at 10.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human TIGIT His Chimera Avi-tag (Catalog # AVI11124) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.

Scientific Data Images for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein Binding Activity

When Recombinant Human CD155/PVR His Protein (2530-CD) is immobilized at 10.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human TIGIT His Chimera Avi-tag Protein (Catalog # AVI11124) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.

Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein (Catalog # AVI11124) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-29 kDa.

Formulation, Preparation and Storage

AVI11124
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). Within the ECD, human TIGIT shares only 65% and 67% aa sequence identity with mouse and rat TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory, and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while in NK cell, TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8). Our Avi-tag Biotinylated Human TIGIT (T103) His-tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. SwissProt Accession # Q495A1.
  6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

Q495A1.1

Additional TIGIT Products

Product Documents for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

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