Recombinant Human TIM-4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9407-TM

R&D Systems, part of Bio-Techne
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9407-TM-050
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Key Product Details

Source

HEK293

Accession #

Q96H15

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TIM-4 protein
Glu25-Gln314, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

32 kDa

SDS-PAGE

77-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.06-0.36 μg/mL.

Scientific Data Images for Recombinant Human TIM-4 His-tag Protein, CF

Recombinant Human TIM-4 His-tag Protein Bioactivity

Recombinant Human TIM-4 His-tag Protein Bioactivity

Recombinant Human TIM-4 (Catalog # 9407-TM) inhibits anti-CD3 antibody-induced proliferation of human T cells. The ED50 for this effect is 0.06-0.36 μg/mL.

Formulation, Preparation and Storage

9407-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5-7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

References

  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Gene Symbol

TIMD4

UniProt

Q96H15

Additional TIM-4 Products

Product Documents for Recombinant Human TIM-4 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TIM-4 His-tag Protein, CF

For research use only

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