Recombinant Human TIMP-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 970-TM

R&D Systems, part of Bio-Techne
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970-TM-010
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Key Product Details

Source

NS0

Accession #

Q6FGX5

Conjugate

Unconjugated

Applications

Inhibition Activity

View all TIMP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TIMP-1 protein
Cys24-Ala207

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

21 kDa

SDS-PAGE

28-30 kDa, reducing conditions

Activity

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The IC50 value is approximately 2.5 nM as measured under the described conditions. 

Reviewed Applications

Read 4 reviews rated 4.5 using 970-TM in the following applications:

Formulation, Preparation and Storage

970-TM
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile, deionized water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol

TIMP1

UniProt

Q6FGX5

Additional TIMP-1 Products

Product Documents for Recombinant Human TIMP-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TIMP-1 Protein, CF

For research use only

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