Recombinant Human TINAGL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9304-TU

R&D Systems, part of Bio-Techne
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9304-TU-050
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Key Product Details

Source

CHO

Accession #

Q9GZM7

Conjugate

Unconjugated

Applications

Bioactivity

View all TINAGL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TINAGL1 protein
Human TINAGL1
(Ala22-Met464)
Accession # Q9GZM7		 HP GGGSGGGSGGGS HHHHHH

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

52 kDa

SDS-PAGE

47-61 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Human TINAGL1 binds with an ED50 = 1-6 ng/mL.

Scientific Data Images for Recombinant Human TINAGL1 Protein, CF

Recombinant Human TINAGL1 Protein Bioactivity

Recombinant Human TINAGL1 Protein Bioactivity

When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Human TINAGL1 (Catalog # 9304-TU) binds with an ED50 = 1-6 ng/mL.

Formulation, Preparation and Storage

9304-TU
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TINAGL1

TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and Lipocalin 7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. Mature human TINAGL1 contains an SMB (somatomedin B) domain, one vWFC domain, and a nonenzymatic peptidase C1A region (1). Mature human TINAGL1 shares 90% amino acid (aa) identity with mouse TINAGL1. Alternative splicing generates additional isoforms that either lack a portion of the vWFC domain or consist of a portion of the peptidase domain with a 38 aa insertion and flanked by substitutions (2). TINAGL1 is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle, and striated muscle cells (1, 3, 4). It is also expressed in mouse decidual endometrium and blastocysts (5-7). During pregnancy it binds to Laminin-1 and Integrins alpha5 and beta1 and plays a role in supporting fertility (6-8). In the vasculature, TINAGL1 localizes to basal laminae along adrenocortical sinusoidal capillaries where it binds to fibronectin and collagen and supports adrenocortical cell adhesion (9).

References

  1. Wex, T. et al. (2001) Biochemistry 40:1350.
  2. Zhou, B. et al. (2000) J. Am. Soc. Nephrol. 11:658.
  3. Bromme, N.C. et al. (2000) Biochem. Biophys. Res. Commun. 271:474.
  4. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
  5. Sakurai, M. et al. (2014) Zygote 22:259.
  6. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
  7. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
  8. Takahashi, A. et al. (2016) J. Reprod. Dev. 62:43.
  9. Li, D. et al. (2007) FEBS J. 274:2506.

Long Name

Tubulointerstitial Nephritis Antigen-like 1

Alternate Names

GIS5, LCN7, LIECG3, OLRG2, P3ECSL, TIN-ag-RP

Entrez Gene IDs

64129 (Human); 94242 (Mouse); 94174 (Rat)

Gene Symbol

TINAGL1

UniProt

Q9GZM7 (Human)

Additional TINAGL1 Products

Product Documents for Recombinant Human TINAGL1 Protein, CF

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Product Specific Notices for Recombinant Human TINAGL1 Protein, CF

For research use only

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