Recombinant Human TLR10 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6619-TR

R&D Systems, part of Bio-Techne
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6619-TR-050
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Key Product Details

Source

CHO

Accession #

Q9BXR5

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TLR10 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TLR10 protein
Human TLR10
(Asp20-Thr576)
Accession # Q9BXR5		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

91.2 kDa (monomer)

SDS-PAGE

115-135 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TLR10 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human TLR1 Fc Chimera (Catalog # 1484-TR) that produces 50% of the optimal binding response is approximately 0.5‑3 µg/mL.

Formulation, Preparation and Storage

6619-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR10

TLR10 (Toll‑like receptor 10, designated CD290) is a 90‑100 kDa type I transmembrane glycoprotein that is a member of the TLR1 subfamily within the TLR family of pathogen-associated molecular pattern (PAMP) receptors (1‑3). Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence, an extracellular domain (ECD, aa 20‑576) with 15 or more leucine‑rich repeats (LRR), an LRRCT (LRR C‑terminal) domain and nine potential sites for N‑linked glycosylation, a transmembrane domain, and a cytoplasmic region (aa 598‑811) with a TIR (Toll/IL‑1 receptor) domain (1, 3). Human TLR10 shares 71%, 81%, 82% and 83% aa sequence identity with rat, bovine, porcine and canine TLR10, respectively. The mouse TLR10 gene is nonfunctional (2). TLR10 is expressed on B cells and cell lines, except for early pre‑B cells, and is more highly expressed on activated than resting cells (2, 4). It is also expressed on FOXP3+ regulatory T cells, placental trophoblasts, and CD1a+ and plasmacytoid dendritic cells (DC), but not on monocyte‑derived DC (2, 5, 6). Among PAMPs, TLR10 is thought to recognize triacylated bacterial lipoproteins (as demonstrated by recognition of the synthetic molecule Pam3CSK4), gram positive bacterial peptidoglycans, and other microbially-derived agonists shared with TLR1 (3, 6, 7). It is found as a homodimer or heterodimer with TLR1 or TLR2, associating through a region within the extracellular LRR repeats (2, 3, 7). It interacts directly with MYD88, leading to production of inflammatory cytokines (2, 7).

References

  1. Chuang, T. and R.J. Ulevitch (2001) Biochim. Biophys. Acta 1518:157.
  2. Hasan, U. et al. (2005) J. Immunol. 174:2942.
  3. Govindaraj, R.G. et al. (2010) PLoS ONE 5:e12713.
  4. Bourke, E. et al. (2003) Blood 102:956.
  5. Bell, M.P. et al. (2007) J. Immunol. 79:1893.
  6. Mulla, M.J. et al. (2013) Am. J. Reprod. Immunol. 69:449.
  7. Guan, Y. et al. (2010) J. Immunol. 184:5094.

Long Name

Toll-like Receptor 10

Alternate Names

CD290

Entrez Gene IDs

81793 (Human)

Gene Symbol

TLR10

UniProt

Q9BXR5

Additional TLR10 Products

Product Documents for Recombinant Human TLR10 Fc Chimera Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human TLR10 Fc Chimera Protein, CF

For research use only

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