Recombinant Human TLR6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7755-TR

R&D Systems, part of Bio-Techne
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7755-TR-050
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Key Product Details

Source

CHO

Accession #

AAY88759

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TLR6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TLR6 protein
Human TLR6
(Asn32-Asn583)
Accession # AAY88759		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn32

Predicted Molecular Mass

89.9 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When peptidoglycan is immobilized at 10 µg/mL (100 µL/well), the concentration of Recombinant Human TLR6 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05‑0.25 μg/mL.

Formulation, Preparation and Storage

7755-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR6

The Toll‑like family of molecules serve as pattern recognition receptors that activate the immune system in response to microbial pathogens. Toll‑like receptors (TLRs) are type I transmembrane proteins in either the plasma membrane or the membranes of endosomal structures. TLRs contain a large number of leucine‑rich repeats (LRRs) in their extracellular domains (ECD) and a cytoplasmic tail with one signal transducing Toll/IL‑1 receptor (TIR) domain (1, 2). Mature human TLR6 is an approximately 115 kDa protein that consists of a 555 amino acid (aa) ECD, a 21 aa transmembrane segment, and a 189 aa cytoplasmic domain (3). Within the ECD, human TLR6 shares 72% aa sequence identity with mouse and rat TLR6. TLR6 is expressed on the surface of macrophages, monocytes, neutrophils, dermal endothelial cells, and alveolar epithelial cells in ligand‑independent association with TLR2 (4‑7). TLR2 also associates with TLR1, creating a functional receptor complex with specificity for related microbial ligands (4, 8, 9). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycans, and glycosylphosphatidylinositols (4‑6, 10‑13). TLR6 plays an important role in the control of Th17 responses and IL‑23 production during allergic inflammation and fungal‑induced asthma (7).

References

  1. Mills, K.H. (2011) Nat. Rev. Immunol. 11:807.
  2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  3. Takeuchi, O. et al. (1999) Gene 231:59.
  4. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
  5. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  6. Bulut, Y. et al. (2001) J. Immunol. 167:987.
  7. Moreira, A.P. et al. (2011) J. Clin. Invest. 121:4420.
  8. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
  9. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  10. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
  11. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
  12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  13. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.

Long Name

Toll-like Receptor 6

Alternate Names

CD286

Entrez Gene IDs

10333 (Human); 21899 (Mouse); 305353 (Rat)

Gene Symbol

TLR6

UniProt

AAY88759

Additional TLR6 Products

Product Documents for Recombinant Human TLR6 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human TLR6 Fc Chimera Protein, CF

For research use only

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