Recombinant Human TMEM119 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10313-TM
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human TMEM119 protein
| Human TMEM119 (Arg26-Met96) Accession # Q4V9L6.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg26
Predicted Molecular Mass
34 kDa
SDS-PAGE
37-57 kDa, under reducing conditions.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Recombinant Human TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Scientific Data Images for Recombinant Human TMEM119 Fc Chimera Protein, CF
Recombinant Human TMEM119 Fc Chimera Protein SDS-PAGE
2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 70-110 kDa, respectively.Formulation, Preparation and Storage
10313-TM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TMEM119
TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimers disease (3). Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).
References
- Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
- Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
- Satoh, J. et al. (2016) Neuropathol. 36:39.
- Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
Long Name
Transmembrane Protein 119
Alternate Names
OBIF
Gene Symbol
TMEM119
UniProt
Additional TMEM119 Products
Product Documents for Recombinant Human TMEM119 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human TMEM119 Fc Chimera Protein, CF
For research use only
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