Recombinant Human TNF RII/TNFRSF1B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 726-R2

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R&D Systems, part of Bio-Techne
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726-R2-050
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Key Product Details

Source

NS0

Accession #

AAA36755

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TNF RII/TNFRSF1B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TNF RII/TNFRSF1B protein
Human TNF RII
(Leu23-Asp257)
Accession # AAA36755		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

52.6 kDa (monomer)

SDS-PAGE

70 kDa, reducing conditions

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect, in the presence of 0.25 ng/mL of TNF-alpha, is 0.004-0.016 µg/mL.

Formulation, Preparation and Storage

726-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa (1-4). The TNF receptor superfamily is comprised of structurally related receptors that bind to TNF-related ligands and regulate numerous processes such as immune cell activation and apoptosis. Receptors in this superfamily are characterized by the presence of a cysteine-rich region in their extracellular domain (ECD) (1-3, 5). Human TNF RII contains four cysteine-rich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively. Several receptors in the TNF superfamily also contain intracellular death domains (DDs) that recruit caspase-interacting proteins to initiate apoptosis upon ligand binding. Those receptors that lack DDs, like TNF RII, bind TNF Receptor-associated Factors, which transduce signals generated by activation of these receptors (6, 7).

TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells (6, 8-10). TNF RII binds to the membrane-bound forms of TNF-alpha and Lymphotoxin-alpha /TNF-beta ; soluble TNF is thought to signal predominately through TNF RI (7, 11). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappaB-dependent signaling pathways (6, 7, 12-15). However, under certain conditions, TNF RII signaling can induce apoptosis (6). TNF RII also exists as a soluble receptor, which can be generated by proteolytic cleavage of its ECD from the cell surface or by alternative splicing (2, 16). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (17). Polymorphisms of the human TNFR2 gene, which result in increased expression of both membrane-bound and soluble TNF RII, have been associated with several autoimmune diseases including Crohn’s disease, systemic lupus erythematosus, and familial rheumatoid arthritis (6, 17).

References

  1. Dembic, Z. et al. (1990) Cytokine 2:231.
  2. Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
  3. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  4. Loetscher, H. et al. (1990) J. Biol. Chem. 265:20131.
  5. Beltinger, C.P. et al. (1996) Genomics 35:94.
  6. Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
  7. Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
  8. Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
  9. Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
  10. Böcker, W. et al. (2008) J. Mol. Med. 86:1183.
  11. Pennica, D. et al. (1992) J. Biol. Chem. 267:21172.
  12. Mak, T.W. and W.C. Yeh (2002) Arthritis Res. 4:S243.
  13. Aspalter, R.M. et al. (2003) J. Leukoc. Biol. 74:572.
  14. Bradley, J.R. (2008) J. Pathol. 214:149.
  15. McCoy, M.K. and M.G. Tansey (2008) J. Neuroinflammation 5:45.
  16. Lainez, B. et al. (2004) Int. Immunol. 16:169.
  17. Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

AAA36755

Additional TNF RII/TNFRSF1B Products

Product Documents for Recombinant Human TNF RII/TNFRSF1B Fc Chimera Protein, CF

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