Recombinant Human TRACP/PAP/ACP5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3948-AP
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TRACP/PAP/ACP5 protein
Ala22-Pro320, with a C-terminal 6-His tag
Ala22-Pro320, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala22
Predicted Molecular Mass
34 kDa
SDS-PAGE
36-40 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >65,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >65,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3948-AP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Do not freeze.
|
Background: TRACP/PAP/ACP5
Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5) (1). The deuced amino acid (aa) sequence of human ACP5 predicts a signal peptide (aa 1 to 21) and a mature chain (aa 22 to 325). R&D Systems’ recombinant human ACP5 consists of aa 22 to 320, without the last 5 residues, RRARP.
ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells (2). Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer (3).
References
- Janckila, A.J. and J.M. Halleen (2003) J. Bone Miner. Res. 18:1892.
- Halleen, J.M. et al. (2006) Clin. Lab. 52:499.
- Janckila, A.J. et al. (2003) J. Bone Miner. Res. 18:1916.
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
UniProt
Additional TRACP/PAP/ACP5 Products
Product Documents for Recombinant Human TRACP/PAP/ACP5 Protein, CF
Product Specific Notices for Recombinant Human TRACP/PAP/ACP5 Protein, CF
For research use only
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