Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10140-T2

R&D Systems, part of Bio-Techne
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10140-T2-100
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Key Product Details

Source

CHO

Accession #

AAH01281.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TRAIL R2/TNFRSF10B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein
Human TRAIL R2/TNFRSF10B
(Ala54-Ser210)
Accession # AAH01281.1
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala54

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-61 kDa, under reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 0.75-6 ng/mL.

Scientific Data Images for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 0.75-6 ng/mL.

Formulation, Preparation and Storage

10140-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).

References

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

AAH01281.1

Additional TRAIL R2/TNFRSF10B Products

Product Documents for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

For research use only

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