Recombinant Human Transglutaminase 2/TGM2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4376-TG
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 2/TGM2 protein
Ala2-Ala687 (Asn533Thr and Leu655Val), with an N-terminal Met and 6-His tag
Ala2-Ala687 (Asn533Thr and Leu655Val), with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained
Predicted Molecular Mass
78 kDa
SDS-PAGE
82 kDa, reducing conditions
Activity
Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.
The specific activity is >1000 pmol/min/ug, as measured under the described condition.
The specific activity is >1000 pmol/min/ug, as measured under the described condition.
Formulation, Preparation and Storage
4376-TG
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein‑glutamine‑ gamma-glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).
References
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Alternate Names
TGC, TGM2, Transglutaminase 2, tTG
Gene Symbol
TGM2
UniProt
Additional Transglutaminase 2/TGM2 Products
Product Documents for Recombinant Human Transglutaminase 2/TGM2 Protein, CF
Product Specific Notices for Recombinant Human Transglutaminase 2/TGM2 Protein, CF
For research use only
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