Recombinant Human Trappin-2/Elafin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1747-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trappin-2/Elafin protein
Ala23-Gln117, with a C-terminal 10-His tag
Ala23-Gln117, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala23
Predicted Molecular Mass
11 kDa
SDS-PAGE
13 kDa and 16 kDa, reducing conditions
Activity
Measured by its ability to inhibit neutrophil elastase cleavage of the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC).
The IC50 value is <50 nM, as measured under the described conditions.
The IC50 value is <50 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1747-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trappin-2/Elafin
Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin‑derived anti‑leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.
References
- Schalkwijk, J. et al. (1999) Biochem. J. 340:569.
Long Name
Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
Alternate Names
Elafin, ESI, PI3, SKALP, Trappin2
Entrez Gene IDs
5266 (Human)
Gene Symbol
PI3
UniProt
Additional Trappin-2/Elafin Products
Product Documents for Recombinant Human Trappin-2/Elafin Protein, CF
Product Specific Notices for Recombinant Human Trappin-2/Elafin Protein, CF
For research use only
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