Recombinant Human TREM2 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1828

Biotinylated
R&D Systems, part of Bio-Techne
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AVI1828-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

NP_061838.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all TREM2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TREM2 protein
Human TREM-2
(His19-Ser174)
Accession # Q9NZC2.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His19

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

60-70 kDa, under reducing conditions.

Activity

When Biotinylated Recombinant Human TREM-2 Fc Chimera Avi-tag is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds fluorescein-conjugated E. coli Bioparticles with an ED50 of 0.100-0.600 µg/mL.

Scientific Data Images for Recombinant Human TREM2 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human TREM2 Fc Chimera Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human TREM-2 Fc Chimera Avi-tag Protein (Catalog # AVI1828) is immobilized onto Streptavidin coated plate (CP004), it binds fluorescein-conjugated E. coli Bioparticles with an ED50 of 0.100-0.600 µg/mL.

Biotinylated Recombinant Human TREM2 Fc Chimera Avitag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TREM2 Fc Chimera Avitag Protein (Catalog # AVI1828) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

AVI1828
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

Triggering Receptor Expressed on Myeloid cells-2 (TREM2) is a type I transmembrane member of the TREM subfamily within the much larger Ig superfamily. In humans, there are 7 TREM and TREM-like receptors which play important roles in the regulation of both innate and adaptive immune response (1). Mature humanTREM2 consists of an extracellular domain (ECD) with one V-type Ig-like domain, a transmembrane domain with a conserved positively-charged lysine residue, and a short cytoplasmic tail (1). The ECD of human TREM2 shares 73% amino acid sequence identity with mouse TREM2. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes (2-6). It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria (6-8). TREM2 associates with the signaling adapter protein DAP12, both preferentially expressed in microglia, to modulate cytokine production (2). Additionally in the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination (3-5, 9). TREM2 also interacts with and modifies the signaling of Plexin A1 on dendritic cells and osteoclasts (10). Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts (11, 12). Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS (13, 14). Our Avi-tag Biotinylated TREM2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Klesney-Tait, J. et al. (2006) Nat Immunol. 7:1266.
  2. Turnbull, I.R. et al. (2006) J. Immunol. 177:3520.
  3. Takahashi, K. et al. (2005) J. Exp. Med. 201:647.
  4. Atagi, Y. et al. (2015) J. Biol. Chem. 290:26043.
  5. Wang, Y. et al. (2016) J. Exp. Med. 213:667.
  6. Cella, M. et al. (2003) J. Exp. Med. 198:645.
  7. Park, M. et al. (2015) Diabetes 64:117.
  8. N'Diaye, E-N. et al. (2009) J. Cell Biol. 184:215.
  9. Poliani, P.L. et al. (2015) J. Clin. Invest. 125:2161.
  10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
  11. Colonna, M. and Y. Wang (2016) Nat. Rev. Neurosci. 17:201.
  12. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
  13. Piccio, L. et al. (2008) Brain 131:3081.
  14. Piccio, L. et al. (2007) Eur. J. Immunol. 37:1290.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

NP_061838.1 (Human)

Additional TREM2 Products

Product Documents for Recombinant Human TREM2 Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TREM2 Fc Chimera Avi-tag Protein, CF

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