Recombinant Human TrkA Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 175-TK

R&D Systems, part of Bio-Techne
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175-TK-050
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Key Product Details

Source

NS0

Accession #

AAA36770

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TrkA Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TrkA protein
Human TrkA
(Ala33-Glu407)
Accession #AAA36770		 IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala33

Predicted Molecular Mass

67.4 kDa (monomer)

SDS-PAGE

115-125 kDa, reducing conditions

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells.
The ED50 for this effect is 0.8-4 µg/mL in the presence of 10 ng/mL of rhNGF.

Formulation, Preparation and Storage

175-TK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References

  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

AAA36770

Additional TrkA Products

Product Documents for Recombinant Human TrkA Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human TrkA Fc Chimera Protein, CF

For research use only

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