Recombinant Human TROP-2 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 650-T2
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TROP-2 protein
| Human TROP-2 (Thr88-Thr274) Accession # P09758.3 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr88
Predicted Molecular Mass
49.2 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells or LS180 human colorectal adenocarcinoma cells.
When 1 x 104 to 5 x 104 cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10 µg/mL with 100 µL/well), >45% cells will adhere after 1 hour incubation.
Optimal dilutions should be determined by each laboratory for each application.
When 1 x 104 to 5 x 104 cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10 µg/mL with 100 µL/well), >45% cells will adhere after 1 hour incubation.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
650-T2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TROP-2
Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.
References
- Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
- Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
- Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
- Ripani, E. et al. (1998) Int. J. Cancer 76:671.
- El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Alternate Names
GA733-1, gp50, T16, TACSTD2, TROP2
Gene Symbol
TACSTD2
UniProt
Additional TROP-2 Products
Product Documents for Recombinant Human TROP-2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human TROP-2 Fc Chimera Protein, CF
For research use only
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