Recombinant Human TROP-2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11158-T2

R&D Systems, part of Bio-Techne
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11158-T2-100
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Key Product Details

Source

CHO

Accession #

P09758.3

Conjugate

Unconjugated

Applications

Bioactivity

View all TROP-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TROP-2 protein
His27-Thr274, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His27

Predicted Molecular Mass

29 kDa

SDS-PAGE

32-57 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGF-II R/IGF2R (Catalog # 6418-GR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human TROP-2 His-tag (Catalog # 11158-T2) binds with an ED50 of 20.0-200 ng/mL.

Scientific Data Images for Recombinant Human TROP-2 His-tag Protein, CF

Recombinant Human TROP-2 His-tag Protein Binding Activity.

When Recombinant Human IGF-II R/IGF2R Protein (6418-GR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human TROP-2 His-tag Protein (Catalog # 11158-T2) binds with an ED50 of 20.0-200 ng/mL.

Recombinant Human TROP-2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human TROP-2 His-tag Protein (Catalog # 11158-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-57 kDa.

Formulation, Preparation and Storage

11158-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is transducing an intracellular calcium signal and may play a role in tumor growth. TROP-2 is associated with EGF mutation and resistance to drug treatment in many tumors. It has been discovered to bind to IGF IIR and promote IGF2-IGF1R-Akt (6). TROP-2 has emerged as a novel cancer target and a target for antibody-drug conjugates (7). TROP-2 interacts with ADAM-10 and binds E-Cadherin stimulating ADAM-10 mediated cleavage (8).

References

  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
  6. Sun, X. et al. (2021) J. Cancer 12:5310.
  7. Goldenberg, D. M. et al. (2018) Oncotarget. 9:28989.
  8. Guerra, E. et al. (2021) Neoplasia. 23:898.

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Gene Symbol

TACSTD2

UniProt

P09758.3

Additional TROP-2 Products

Product Documents for Recombinant Human TROP-2 His-tag Protein, CF

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Product Specific Notices for Recombinant Human TROP-2 His-tag Protein, CF

For research use only

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