Recombinant Human Tsukushi/TSK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3940-TS

R&D Systems, part of Bio-Techne
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3940-TS-050
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Key Product Details

Source

NS0

Accession #

Q8WUA8

Conjugate

Unconjugated

Applications

Binding Activity

View all Tsukushi/TSK Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Tsukushi/TSK protein
Thr17-Leu353, with a C-terminal 6-His tag

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr17

Predicted Molecular Mass

40 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhBMP-4 at 1 µg/mL can bind rhTSK with an apparent KD <15 nM.

Formulation, Preparation and Storage

3940-TS
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tsukushi/TSK

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (1, 2). All contain leucine-rich repeats (LRR) flanked by conserved cysteines, but the C-terminus of TSK resembles the structure of nyctalopin and chondroadherin rather than class I - III SLRPs (1). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted (presumably glycosylated) protein that contains nine LRR (2). Proteoglycan modification of TSK has not been shown. SLRP-like activity or expression of TSK has been identified in several contexts. TSK mRNA is upregulated by estrogen treatment in breast cancer cell lines, and so is proposed to be involved in hormonally regulated extracellular matrix remodeling (2). TSK is upregulated along with bone markers in Vitamin K2-treated osteosarcoma cell lines, mediated by the pregnane X receptor, PXR (3). TSK was also shown to contribute to vitamin K2-mediated enhancement of collagen accumulation. Other SLRPs have been shown to form a protective coat around collagen fibrils, shielding them from proteolysis (4). In rats, expression of TSK in liver, intestines and islets of Langerhans resembles the pattern of Glut2 (5). TSK transcription is upregulated by both estrogen and insulin in hepatocytes. In Xenopus and chick embryos, TSK is expressed in the middle primitive streak, where it binds and antagonizes BMP-4 and promotes dorsalization and formation of the neural crest and the primitive organizer (6, 7). Xenopus TSK also binds the extracellular region of delta-1, modulating Notch activity (7). Human TSK shows 87%, 86%, 86%, 85%, 52% and 50% aa identity with canine, mouse, rat, bovine, chick, and Xenopus TSK, respectively, with the highest identity in the first four LRR.

References

  1. McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294.
  2. Charpentier, A.H. et al. (2000) Cancer Res. 60:5977.
  3. Ichikawa, T. et al. 2006, J. Biol. Chem. 281:16927.
  4. Geng, Y. et al. (2006) Matrix Biol. 25:484.
  5. Coffy, S. et al. (2005) Biochem. J. 385:165.
  6. Ohta, K. et al. (2004) Dev. Cell 7:347.
  7. Kuriyama, S. et al. (2006) Development 133:75.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Alternate Names

E2IG4, LRRC54, TSKU

Entrez Gene IDs

25987 (Human)

Gene Symbol

TSKU

UniProt

Q8WUA8

Additional Tsukushi/TSK Products

Product Documents for Recombinant Human Tsukushi/TSK Protein, CF

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Product Specific Notices for Recombinant Human Tsukushi/TSK Protein, CF

For research use only

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