Recombinant Human ULBP-2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11196-UL

R&D Systems, part of Bio-Techne
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11196-UL-050
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Key Product Details

Source

CHO

Accession #

Q9BZM5.1

Conjugate

Unconjugated

Applications

Bioactivity

View all ULBP-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ULBP-2 protein
Gly26-Ser217, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

23 kDa

SDS-PAGE

25-35 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human ULBP-2 Hs--tag (Catalog # 11196-UL) binds with an ED50 of 7.50-60.0 ng/mL.

Scientific Data Images for Recombinant Human ULBP-2 His-tag Protein, CF

Recombinant Human ULBP-2 His-tag Protein Binding Activity.

When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human ULBP-2 His-tag Protein (Catalog # 11196-UL) binds with an ED50 of 7.50-60.0 ng/mL.

Recombinant Human ULBP-2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human ULBP-2 His-tag Protein (Catalog # 11196-UL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 25-35 kDa.

Formulation, Preparation and Storage

11196-UL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-2

ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance.

References

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) www.rndsystems.com.

Long Name

UL16 Binding Protein-2

Alternate Names

ALCAN-alpha, N2DL2, NKG2DL2, RAET1H, ULBP2

Entrez Gene IDs

80328 (Human)

Gene Symbol

ULBP2

UniProt

Q9BZM5.1

Additional ULBP-2 Products

Product Documents for Recombinant Human ULBP-2 His-tag Protein, CF

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Product Specific Notices for Recombinant Human ULBP-2 His-tag Protein, CF

For research use only

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