Recombinant Human uPAR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10378-UK

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

Q03405.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all uPAR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human uPAR protein
Human uPAR
(Leu23-Arg303)
Accession # Q03405.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

58 kDa

SDS-PAGE

74-85 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE) binds with an ED50 of 2-16 ng/mL.

Scientific Data Images for Recombinant Human uPAR Fc Chimera Protein, CF

Recombinant Human uPAR Fc Chimera Protein Binding Activity

Recombinant Human uPAR Fc Chimera Protein Binding Activity

When Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) binds with an ED50 of 2-16 ng/mL.
Recombinant Human uPAR Fc Chimera Protein SDS-PAGE

Recombinant Human uPAR Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa and 150- 170 kDa, respectively.

Formulation, Preparation and Storage

10378-UK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

uPAR (urokinase-type plasminogen activator receptor, also known as CD87), is a cell surface receptor that binds urokinase-type plasminogen activator (uPA) which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPAR expression is activated during inflammation, immune responses, and conditions of tissue remodeling and wound healing (1). Human uPAR is synthesized as a 335 amino acid (aa) residue precursor protein with a 22 aa signal peptide. Removal of the C-terminal propeptide generates a fully processed uPAR containing 283 aa with five potential N-linked glycosylation sites and a C-terminal GPI-anchor site (2). Variants due to alternative splicing of uPAR also exist (3). Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific (4). The uPA/uPAR interaction initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR is considered as a biomarker in many inflammatory diseases including cancer, cardiovascular diseases, chronic kidney diseases and diabetes (5).

References

  1. Smith, H.W. and C.J. Marshall (2010) Nat. Rev. Mol. Cell Biol. 11:23.
  2. Ploug, M. et al. (1991) J. Biol. Chem. 266:1926.
  3. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
  4. Ellis, V. et al. (1992) Ann. N.Y. Acad. Sci. 667:13.
  5. Desmedt, S. et al. (2017) Crit. Rev. Clin. Lab. Sci. 54:117.

Long Name

Urokinase-type Plasminogen Activator Receptor

Alternate Names

PLAUR

Entrez Gene IDs

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Gene Symbol

PLAUR

UniProt

Q03405.1

Additional uPAR Products

Product Documents for Recombinant Human uPAR Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human uPAR Fc Chimera Protein, CF

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