Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10139-VN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q6EMK4

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Vasorin/SLIT-like 2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Vasorin/SLIT-like 2 protein
Human Vasorin/SLIT-like 2
(Cys24-Asn573)
Accession # Q6EMK4		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

86 kDa

SDS-PAGE

106-120 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TGF‑ beta1 (Catalog # 240-B) is used at 1 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT‑like 2 Fc Chimera (Catalog# 10139-VN) binds with an ED50 of 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein, CF

Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein Binding Activity

Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein Binding Activity

When Recombinant Human TGF-beta 1 (Catalog # 240-B) is used at 1 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT-like 2 Fc Chimera (Catalog # 10139-VN) binds with an ED50 of 0.4-2.4 µg/mL.
Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein SDS-PAGE

Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Vasorin/SLIT-like 2 Fc Chimera (Catalog # 10139-VN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-120 kDa and 220-240 kDa, respectively.

Formulation, Preparation and Storage

10139-VN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110-kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95-kDa soluble form of Vasorin can be shed from the cell surface by ADAM17-mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta-induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature human Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type III domain, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (1). Within the ECD, human Vasorin shares 85% and 86% aa sequence identity with mouse and rat Vasorin, respectively.

References

  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
  2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
  3. Malapeira, J. et al. (2011) Oncogene 30:1912.
  4. Li, S. et al. (2015) Oncotarget 6:10045.

Alternate Names

Atia, SLIT like 2, Slitl2, VASN

Entrez Gene IDs

114990 (Human); 246154 (Mouse)

Gene Symbol

VASN

UniProt

Q6EMK4

Additional Vasorin/SLIT-like 2 Products

Product Documents for Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Vasorin/SLIT-like 2 Fc Chimera Protein, CF

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