Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11508

Fc Chimera, biotinylated
R&D Systems, part of Bio-Techne
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AVI11508-100
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Key Product Details

Source

CHO

Accession #

P19320.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VCAM-1/CD106 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein
Human VCAM-1
(Phe25-Glu698)
Accession # P19320.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe25

Predicted Molecular Mass

103 kDa

SDS-PAGE

105-130 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human VCAM 1/CD106 Fc Chimera Avi-tag (Catalog # AVI11508) binds Recombinant Human Integrin alpha4 beta1 (Catalog # 5668-A4) with an ED50 of 7.50-90.0 ng/mL.

Scientific Data Images for Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) binds Recombinant Human Integrin alpha4 beta1 (5668-A4) with an ED50 of 7.50-90.0 ng/mL.

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-130 kDa, and 210-260 kDa, respectively.

Formulation, Preparation and Storage

AVI11508
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha4 beta1 (VLA-4) and alpha4 beta7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. Our Avi-tag Biotinylated human VCAM-1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

UniProt

P19320.1

Additional VCAM-1/CD106 Products

Product Documents for Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

For research use only

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