Recombinant Human VEGF 189 (aa 27-215) Protein

R&D Systems, part of Bio-Techne | Catalog # 8147-VE

R&D Systems, part of Bio-Techne
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Carrier Free
8147-VE-025/CF
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With Carrier
8147-VE-025
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Key Product Details

Source

CHO

Accession #

AAA36804

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VEGF protein
Ala27-Arg215

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

22 kDa (monomer)

SDS-PAGE

22-30 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.
The ED50 for this effect is 4-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8147-VE
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8147-VE/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

VEGF189 (Vascular Endothelial Growth Factor-A of 189 amino acids) is a 50-52 kDa homodimeric glycoprotein member of the PDGF/VEGF family of molecules (1, 2). A large number of human VEGF isoforms are generated by alternative splicing, including VEGF189 which lacks amino acids (aa) 166-182 of the full length protein (VEGF206) (3-5). Mature human VEGF189 shares 88% aa sequence identity with mouse VEGF188. VEGF189 binds to heparan sulfate both on the cell surface and in the extracellular matrix (ECM) where it retains bioactivity (6, 7). It can undergo proteolytic cleavage from the ECM (at Arg136-Ala137), creating a soluble fragment with increased bioactivity (6, 7). VEGF189 is widely expressed including by vascular smooth muscle cells (3), chondrocytes (8), fibroblasts (9), neuronal and glial cells (10), and neutrophils (11). VEGF189 interacts with various receptors including VEGF R1, VEGF R2, Neuropilin-1, and Integrins aVb3, aVb5, a3b1, and a5b1 (6, 12-14). VEGF189 exerts complex effects in angiogenesis. It is proapoptotic towards quiescent vascular endothelium but is proangiogenic towards endothelium that has committed to the angiogenic process (6, 14). In particular, venous endothelial cells are highly responsive towards VEGF189, while the responsiveness of arterial endothelium is primarily restricted to the regulation of migration (7). In addition to its vascular effects,  immobilized VEGF189 serves as an anchor for cells expressing Integrin avb3, and it acts as both a chemotactic and chemokinetic factor for migrating neutrophils (11, 13, 14).

References

  1. Woolard, J. et al. (2009) Microcirculation 16:572.

  2. Ferrara, N. et al. (2003) Nat. Med. 9:669.

  3. Tischer, E. et al. (1991) J. Biol. Chem. 266:11947.

  4. Leung, D.W. et al. (1989) Science 246:1306.

  5. Keck, P.J. et al. (1989) Science 246:1309.

  6. Plouet, J. et al. (1997) J. Biol. Chem. 272:13390.

  7. Herve, M-A. et al. (2005) Exp. Cell Res. 309:24.

  8. Cramer, T. et al. (2004) Osteoarthritis Cartilage 12:433.

  9. Detmar, M. et al. (1997) J. Invest. Dermatol. 108:263.

  10. Tang, H. et al. (2013) Neurobiol. Aging 34:1412.

  11. Houck, K.A. et al. (1992) J. Biol. Chem. 267:26031.

  12. Herve, M-A. et al. (2008) Am. J. Pathol. 172:167.

  13. Hutchings, H. et al. (2003) FASEB J. 17:1520.

  14. Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

AAA36804

Additional VEGF Products

Product Documents for Recombinant Human VEGF 189 (aa 27-215) Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human VEGF 189 (aa 27-215) Protein

For research use only

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