Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT7126

R&D Systems, part of Bio-Techne
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BT7126-050
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Key Product Details

Source

NS0

Accession #

AAH20568

Structure / Form

Disufide-linked homodimer. Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all VISTA/B7-H5/PD-1H Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1H protein
Human VISTA/B7-H5/PD-1H
(Phe33-Ala194)
Accession # AAH20568		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33

Predicted Molecular Mass

45 kDa (unlabeled)

SDS-PAGE

64 - 75 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VSIG-3 Fc Chimera (Catalog # 9229-VS) is immobilized at 5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant VISTA/B7-H5/PD-1H Fc Chimera that produces 50% of the optimal binding response is 1-6 μg/mL.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is typically 1-6 μg/m

Scientific Data Images for Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

Recombinant Human VISTA Fc Chimera Biotinylated Protein Binding Activity

Recombinant Human VISTA Fc Chimera Biotinylated Protein Binding Activity

When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is coated at 5 µg/mL, Biotinylated Recombinant Human VISTA/B7-H5 Fc Chimera (Catalog # BT7126) binds with an ED50 of 1-6 µg/mL.

Formulation, Preparation and Storage

BT7126
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7‑H5, is a 55‑65 kDa transmembrane glycoprotein with homology to B7‑like immune co‑stimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V‑type Ig‑like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1‑MMP, with a 25‑30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1‑MMP expression and the MT1‑MMP mediated activation of MMP‑2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP‑4 induced signaling in ESC, but is also down‑regulated following BMP‑4 exposure (4, 5). It binds to BMP‑4 directly, and also associates with the type I BMP receptor Activin RIB/ALK‑4 (4, 5). Gi24 is expressed on the surface of naïve CD4+ T cells and regulatory T cells (6). It is up‑regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL‑2 and IFN‑ gamma (6). Its expression on tumor cells attenuates the anti‑tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6).

References

  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

AAH20568

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

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