Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9229

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q5DX21.3

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VSIG3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSIG3 protein
Human VSIG3
(Leu23-Gly245)
Accession # Q5DX21.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

52 kDa

SDS-PAGE

62-69 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein (Catalog # 7126-B7) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) that produces 50% of the optimal binding response is found to be approximately 3-15 μg/mL.

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 or IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-10 μg/mL.

Scientific Data Images for Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

Recombinant Human VSIG3 Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human VSIG3 Fc Chimera Avi-tag Protein Binding Activity

When Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein (Catalog # 7126-B7) is immobilized at 2.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) that produces 50% of the optimal binding response is found to be approximately 3-15 µg/mL.
Recombinant Human VSIG3 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human VSIG3 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-69 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

AVI9229
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: VSIG3

VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).

References

  1. Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
  2. Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
  3. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
  4. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
  5. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
  6. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
  7. Watanabe, T. et al. (2005) Cancer Sci. 96:498.
  8. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Alternate Names

BTIGSF, CXADRL1, IGSF11

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Gene Symbol

IGSF11

UniProt

Q5DX21.3

Additional VSIG3 Products

Product Documents for Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

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