Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10704

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

NP_001304772.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VSTM2A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSTM2A protein
Human VSTM2A
(Ser25-Phe244)
Accession # NP_001304772.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25

Predicted Molecular Mass

53 kDa

SDS-PAGE

62-75 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1.00-10.0 μg/mL

Measured by its binding ability in a functional ELISA.
When Human VSTM2A Antibody  (Catalog # MAB100371) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VSTM2A Fc Chimera Avi-tag (Catalog # AV10704) binds with an ED50 of 1.00-8.00 ng/mL.

Scientific Data Images for Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human VSTM2A Fc Chimera Avi-tag Protein Binding Activity

When Human VSTM2A Antibody (MAB100371) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VSTM2A Fc Chimera Avi-tag (Catalog # AV10704) binds with an ED50 of 1.00-8.00 ng/mL

Formulation, Preparation and Storage

AVI10704
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is a member of the immunoglobulin superfamily (1). The VSTM family consists of 8 family members, each containing an immunoglobulin V-set (IgV) domain in the extracellular domain (ECD). In addition to the single IgV domain, VSTM2A contains two N-glycosylation sites in the ECD that are critical for its secretion, but not for activity (2). In humans, two isoforms exist due to alternative splicing, varying in the C-terminal residues. The mature ECD of human VSTM2A, isoform 2 shares 80% amino acid sequence identity with mouse and rat VSTM2A isoform 2. VSTM2A is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A promotes regulation and commitment of white and brown preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 2). VSTM2A is highly expressed in the brain but the role of VSTM2A in neuronal and brain development remain uncharacterized (3). VSTM2A is a critical tumor suppressor in colorectal carcinogenesis and a novel antagonist of Wnt signaling receptor LRP6, and might serve as a new prognostic marker for colorectal cancer patients (4). Our in house data show that VSTM2A inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation. Our Avi-tag Biotinylated VSTM2A features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Secco, B. et al. (2017) Cell Rep. 18:93.
  2. Berry, D.C. et al. (2013) Development. 140:3939.
  3. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
  4. Zhang, D. et al. (2019) Theranostics. 9:6517.

Long Name

V-Set And Transmembrane Domain Containing 2A

Alternate Names

VSTM2

Entrez Gene IDs

222008 (Human); 211739 (Mouse); 689106 (Rat)

Gene Symbol

VSTM2A

UniProt

NP_001304772.1

Additional VSTM2A Products

Product Documents for Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF

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