Recombinant Human VSTM2A His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10037-VT

R&D Systems, part of Bio-Techne
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10037-VT-050
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Key Product Details

Source

HEK293

Accession #

Q8TAG5-2

Conjugate

Unconjugated

Applications

Bioactivity

View all VSTM2A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VSTM2A protein
Ser25-Phe244, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25

Predicted Molecular Mass

25 kDa

SDS-PAGE

38-42 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-10 μg/mL.

Scientific Data Images for Recombinant Human VSTM2A His-tag Protein, CF

Recombinant Human VSTM2A His-tag Protein Bioactivity

Recombinant Human VSTM2A His-tag Protein Bioactivity

Recombinant Human VSTM2A (Catalog # 10037-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.
Recombinant Human VSTM2A His-tag Protein SDS-PAGE

Recombinant Human VSTM2A His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human VSTM2A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-42 kDa.

Formulation, Preparation and Storage

10037-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A. Our in house data show that VSTM2A inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.

References

  1. Secco, B. et al. (2017) Cell Rep. 18:93.
  2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
  3. Berry, D.C. et al. (2013) Development. 140:3939.

Long Name

V-Set And Transmembrane Domain Containing 2A

Alternate Names

VSTM2

Entrez Gene IDs

222008 (Human); 211739 (Mouse); 689106 (Rat)

Gene Symbol

VSTM2A

UniProt

Q8TAG5-2

Additional VSTM2A Products

Product Documents for Recombinant Human VSTM2A His-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human VSTM2A His-tag Protein, CF

For research use only

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