Recombinant Human XCL1/Lymphotactin Protein
R&D Systems, part of Bio-Techne | Catalog # 695-LT
Key Product Details
Product Specifications
Source
E. coli-derived human XCL1/Lymphotactin protein
Val22-Gly114, with an N-terminal Met
Val22-Gly114, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
10.4 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human XCR1.
The ED50 for this effect is 0.0800-0.500 µg/mL.
The ED50 for this effect is 0.0800-0.500 µg/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 695-LT
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 695-LT/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: XCL1/Lymphotactin
Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4+ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.
References
- Kennedy, J. et al. (1995) J. Immunol. 155:203.
- Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
UniProt
Additional XCL1/Lymphotactin Products
Product Documents for Recombinant Human XCL1/Lymphotactin Protein
Product Specific Notices for Recombinant Human XCL1/Lymphotactin Protein
For research use only
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