Recombinant Human XIAP (BIR3) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 895-XB
Key Product Details
Product Specifications
Source
E. coli-derived human XIAP protein
Asn252-Thr356, with an N-terminal Met and 6-His tag
Asn252-Thr356, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
13 kDa
SDS-PAGE
11 kDa, reducing conditions
Activity
Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The IC50 for this effect is <300 nM.
Optimal dilutions should be determined by each laboratory for each application.
The IC50 for this effect is <300 nM.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
895-XB
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: XIAP
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit caspases. The BIR3 domain inhibits caspase-9. The ability of XIAP (BIR3 domain) to inhibit caspases is prevented by SMAC/Diablo.
References
- Deveraux, Q. et al. (1998) EMBO J. 17(8):2215.
- Deveraux, Q. et al. (1999) EMBO J. 18(19):5242.
- Verhagen, A. et al. (2000) Cell 102:43.
- Chai, J. et al. (2001) Cell 104:769.
- Riedl, S. (2001) Cell 104:791.
- Suzuki, Y. (2001) J. Biol. Chem. 276(29):27058.
- Ekert, P. et al. (2001) J. Cell Biol. 152(3):483.
- Du, C. et al. (2000) Cell 102:33.
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Recombinant Human XIAP (BIR3) Protein, CF
Product Specific Notices for Recombinant Human XIAP (BIR3) Protein, CF
For research use only
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